Method for detecting a nucleotide sequence

US6403339B1 · kind B1 · utility

Assignee

• november Aktiengesellschaft Gesellschaft fur Molekulare Medizin · DE

Inventor

• Wolf Bertling · Erlangen, DE

Key dates

• Filing date: Nov 16, 2000
• Grant date: Jun 11, 2002
• Expiry date: Nov 16, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6818
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method is disclosed for detecting a nucleotide sequence by a polymerase chain reaction, the nucleotide sequence is a constituent of a double-stranded nucleic acid molecule which is in solution and formed of a strand and a counter-strand. A first primer is added to the solution and is complementary to a first segment of the strand located at the 5′-terminus of a central segment. A second primer is added and is complementary to a 5′-terminal second segment of the counter-strand. The solution is brought into contact with a third primer whose 5′-terminal end is bound to a solid phase. The third primer is complementary to the central segment of the strand containing the nucleotide sequence. A reaction vessel is closed which contains the solution. The solution is repeatedly heated and cooled and the nucleic acid molecule is detected in the closed reaction vessel.