Method for cloning and expression of BstYI restriction endonuclease and BstYI methylase in E. coli and purification of BstYI and M.BstYI enzymes
US6403354B1 · kind B1 · utility
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6Claims
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Key dates
| Filing date | Jan 19, 2001 |
| Grant date | Jun 11, 2002 |
| Priority date | — |
| Expiry date | Jan 19, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to recombinant DNA which encodes the BstYI restriction endonuclease as well as BstYI methyltransferase, expression of BstYI restriction endonuclease and M.BstYI in E. coli cells containing the recombinant DNA. It also relates to methods for purification of the recombinant BstYI restriction endonuclease and BstYI methyltransferase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.