Method of producing transgenic maize using direct transformation of commercially important genotypes

US6403865B1 · kind B1 · utility

Assignee

• Syngenta Investment Corporation · US

Inventors

• Michael G. Koziel · Clive, US
• Nalini M. Desai · Cary, US
• Kelly S. Lewis · Hillsborough, US
• Vance Cary Kramer · Hillsborough, US
• Gregory W. Warren · Cary, US
• Stephen V. Evola · Apex, US
• Lyle D. Crossland · Chapel Hill, US
• Martha S. Wright · Cary, US
• Ellis J. Merlin · Raleigh, US
• Karen L. Launis · Wake Forest, US
• Steven Rothstein · Clive, US
• Cindy G. Bowman · Cary, US
• John L. Dawson · Chapel Hill, US
• Erik Dunder · Chapel Hill, US
• Gary M. Pace · Cary, US
• Janet L. Suttie · Raleigh, US
• Nadine Carozzi · Raleigh, US
• Annick Jeanne De Framond · Durham, US
• James Linder · Owatonna, US
• Robert Lynn Miller · Iowa City, US
• Bruce Skillings · Innerkip, CA
• Alan Mousel · Bluffton, US
• Albert R. Hornbrook · Normal, US
• Christopher Clucas · Rochelle, US
• Moez Meghji · Bloomington, US
• Andreas Tanner · Plaisance-du-Touch, FR
• Francis Cassagne · Auch, FR
• Gilles Pollini · Saint-Gaudens, FR
• Terry R. Colbert · Dyersburg, US
• Francis Cammack · Rochelle, US

Key dates

• Filing date: May 10, 1995
• Grant date: Jun 11, 2002
• Expiry date: May 10, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.