Method for preparation of microprojectiles for efficient delivery of biologicals using a particle gun
US6406852B1 · kind B1 · utility
1Cited by
1References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 22, 2000 |
| Grant date | Jun 18, 2002 |
| Priority date | — |
| Expiry date | Jun 22, 2020 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K48/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a process for uniform and efficient delivery of metal projectiles into living cells and tissue of plants and animals, by bombarding the cells with biological coated on metal bead particles. The metal bead particles are heated in dry oven to high temperature, prior to coating with DNA and by substituting ethanol with isopropanol while coating the particles with DNA, to give several fold higher expression of the nucleic acid delivered into cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.