Fluorescent capture assay for kinase activity employing anti-phosphotyrosine antibodies as capture and detection agents
US6406869B1 · kind B1 · utility
24Cited by
7References
12Claims
0Family size
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Key dates
| Filing date | Oct 22, 1999 |
| Grant date | Jun 18, 2002 |
| Priority date | — |
| Expiry date | Oct 22, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/91235
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining the level of tyrosine kinase activity in a biological sample is disclosed. The method employs an anti-phosphotyrosine antibody as both the capture agent and the detecting agent. The detecting antibody is labeled with a lanthanide ion, such as europium, as the signal generating entity. The method is particularly well suited to high throughput screening, for example, for compounds which modulate tyrosine kinase activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.