Chimeric proteins with a cellulose binding domain
US6407208B1 · kind B1 · utility
Inventors
Key dates
| Filing date | Oct 6, 1998 |
| Grant date | Jun 18, 2002 |
| Priority date | — |
| Expiry date | Oct 6, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Chimeric proteins containing a cellulose binding domain (CBD) situated between two independent desired proteins of different lengths can be contemplated for use in a wide range of applications. In addition to its affinity to cellulose due to the presence of the CBD, various properties can be adopted to the chimeric protein by means of introducing the desired proteins upstream and downstream of the CBD. For example, a chimeric protein is produced in Escherichia coli in large quantities by joining a thioredoxin, a CBD and a short peptide composed of 3 amino acid (i.e., Arginine-Glutamate-Asparate). This chimeric protein can be further purified in simple steps and added to cell culture to enhance cell attachment to a surface made up of cellulose.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.