Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
US6410317B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Jul 14, 2000 |
| Grant date | Jun 25, 2002 |
| Priority date | — |
| Expiry date | Nov 6, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/203
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for producing an expression vector. In the subject methods, donor and acceptor vectors are combined in the presence of a recombinase to produce an expression vector that includes a first and second recombinase recognition site oriented in the same direction, wherein the first and second recombination sites are able to recombine with each other. In the subject methods, one of the donor and acceptor vectors includes a single recombinase recognition site while the other includes two recombinase recognition sites. Also provided are compositions for use in practicing the subject methods, including the donor and acceptor vectors themselves, as well as systems and kits that include the same. The subject invention finds use in a variety of different applications, including the transfer or cloning of a nucleic acid of interest from a first vector into one or more expression vectors, etc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.