Method for electrochemical detection

US6413398B1 · kind B1 · utility

Assignee

• Her Majesty the Queen in Right of Canada, as represented by the Canadian Food Inspection Agency · CA

Inventors

• Geoff C. Gerhardt · Fairhaven, US
• Richard Murray Cassidy · La Ronge, CA
• Andrzej Baranski · Guelph, CA

Key dates

• Filing date: Sep 13, 1999
• Grant date: Jul 2, 2002
• Expiry date: Sep 13, 2019

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/4473
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for electrochemical detection (ECD) is disclosed. The illustrated embodiments are particularly adapted to use in detection of analytes separated by capillary electrophoresis. The method is a voltammetric ECD, including a preparatory positive potential pulse, an optional stabilizing pulse at a negative potential, an analytical pulse at extreme negative potentials (e.g., −300 mV to −1,500 mV), and a positive cleaning potential pulse. The methods have been shown to detect analytes previously considered to be electrochemically inactive using more traditional electrochemical methods. Examples of such analytes include various drugs, antibiotics, and peptides.