Stabilized reagent and method for determining creatine kinase

US6413733B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Rolf Nagel · Bürstadt, DE
• Juergen Mistele · Zeven, DE
• Norbert Schroeder · Dresden, DE

Key dates

• Filing date: Jun 9, 2000
• Grant date: Jul 2, 2002
• Expiry date: Jun 9, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A stabilized reagent for the photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detecting the ATP formed, containing an organic or inorganic sulphur compound preferably in a submolar amount relative to the added CK activator. A corresponding reagent in a liquid form is stable at 2 to 8° C. for up to 12 months without significant loss of function.