Method of chromatographically purifying or fractionating, respectively, von Willebrand factor from a VWF-containing starting material

US6414125B1 · kind B1 · utility

Assignee

• Baxter Aktiengesellschaft · AT

Inventors

• Juergen Siekmann · Wien, AT
• Peter Turecek · Klosterneuburg, AT
• Hans-Peter Schwarz · Wien, AT
• Johann Eibl · Wien, AT
• Bernhard Fischer · Wien, AT
• Artur Mitterer · Orth an der Donau, AT
• Friedrich Dorner · Wien, AT

Key dates

• Filing date: Oct 21, 1999
• Grant date: Jul 2, 2002
• Expiry date: Oct 21, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/755
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed is a method of chromatographically purifying or fractionating, respectively, von Willebrand factor (vWF) from a vWF-containing starting material, comprising the following steps:adsorbing the vWF from the starting material on avid collagen immobilized on a carrier,separating the non-adsorbed portion and, optionally, washing the carrier,eluting the vWF from immobilized collagen, andrecovering the purified vWF, as well as a pharmaceutical preparation comprising biologically active vWF which is bound to collagen in a stable manner.