Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
US6414136B1 · kind B1 · utility
8Cited by
9References
38Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 3, 2000 |
| Grant date | Jul 2, 2002 |
| Priority date | — |
| Expiry date | May 3, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods for removing unincorporated fluorescent dye-labeled molecules from a mixture that includes fluorescently-labeled polynucleotides and the unincorporated fluorescent dye-labeled molecules. The methods involve adsorbing the unincorporated fluorescent dye-labeled molecules into a plurality of particles that are made up of one or more porous hydrophobic materials that are encapsulated in a hydrophilic matrix.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.