Chimeric polyclonal antibodies
US6420113B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 2, 1999 |
| Grant date | Jul 16, 2002 |
| Priority date | — |
| Expiry date | Oct 2, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2400/50
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is directed to production of chimeric antibodies using display screening methods. The invention is based in part on two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods can be used to produce polyclonal libraries of chimeric antibodies for use, e.g., as diagnostic or therapeutic reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.