Nucleotide sequences which code for the pck gene

US6420151B1 · kind B1 · utility

Assignee

• Degussa-Huels AG · DE

Inventors

• Bernard Eikmanns · Ulm, DE
• Christian Riedel · Munich, DE
• Hermann Sahm · Jülich, DE
• Bettina Mockel · Bielefeld, DE

Key dates

• Filing date: Dec 7, 1999
• Grant date: Jul 16, 2002
• Expiry date: Dec 7, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/08
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence chosen from the group consisting ofa) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2,b) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for the polypeptide which is expressed by the pck gene contained on vector pK19mobsacB&Dgr;pck in the deposited E.coli strain DSM 13047,c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2,d) polynucleotide which is complementary to the polynucleotides of a), b) or c) ande) polynucleotide comprising at least 15 successive bases of the polynucleotide sequence of a), b), c) or d).