Purified proteolytic enzyme and method of purification
US6420156B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 17, 1998 |
| Grant date | Jul 16, 2002 |
| Priority date | — |
| Expiry date | Aug 17, 2018 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A purified protease preparation of chymotrypsin and porcine trypsin is prepared by adjusting the pH of a protease solution comprising chymotrypsin and porcine trypsin to a value of between 6 and 9, maintaining the solution at this pH and at 20-35° C. for at least 15 minutes to at most 120 minutes, so as to allow the proteolytic activity of the proteases to destroy the lipolytic activity of the lipases and phospholipases in the solution. Subsequently, the pH of the solution is reduced to a value of less than or equal to 3.5. The purified protease preparation allows the manufacture of infant formulae containing lecithin which are stable during storage and do not exhibit significant degradation of the added lecithin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.