Masking background fluorescence and luminescence in optical analysis of biomedical assays

US6420183B1 · kind B1 · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Thoams Krahn · Hagen, DE
• Wolfgang Paffhausen · Leverkusen, DE
• Andreas Schade · Lengede, DE
• Martin Bechem · Wuppertal, DE
• Delf Schmidt · Wuppertal, DE

Key dates

• Filing date: Nov 20, 1998
• Grant date: Jul 16, 2002
• Expiry date: Nov 20, 2018

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/13
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. The separating layer 10 must be composed such that it has a high power of reflection for the luminescent light 11. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer 12 at the bottom 2 of a reaction vessel 1 is in contact with a solution in which a fluorescent or luminescent ligand 13 is dissolved. The …