Xylanase derived from a bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

US6423523B1 · kind B1 · utility

Assignee

• GENENCOR INTERNATIONAL, INC. · US

Inventors

• Eric De Buyl · Sint-Genesius-Rode, BE
• Andrée Lahaye · Brussels, BE
• Pierre Ledoux · Brussels, BE
• Antoine Amory · Rixensart, BE
• Rene Detroz · Waterloo, BE
• Christophe Andre · Laissac, FR
• Roman Vetter · Burgdorf, DE

Key dates

• Filing date: May 12, 1998
• Grant date: Jul 23, 2002
• Expiry date: May 12, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A purified xylanase derived from B. Pumilus PRL B12 is disclosed. This xylanase is efficient for use in the biobleaching of wood pulp, permitting a strong reduction in the quantity of chlorine used and AOX compounds produced in classical and ECF wood pulp bleaching sequences as well as the quantity of ozone used in TCF sequences. The gene coding for the xylanase was isolated and purified and used to construct an expression vector therefor. A recombinant host strain of B. licheniformis is also disclosed which is efficient for expressing heterologous enzymes, including the xylanase when transformed by the expression vector.