Method for purifying biological marcromolecules, and chromatographic column for performing said method
US6428703B1 · kind B1 · utility
12Cited by
8References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 23, 1999 |
| Grant date | Aug 6, 2002 |
| Priority date | — |
| Expiry date | Dec 23, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/34
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention concerns a method for purifying biological macromolecules, e.g. nucleic acids or proteins, from starting materials, in which the macromolecules, contained in a biological solution, are purified on a chromatographic material, and in which endotoxins are partially or completely removed with the aid of a nonionic surfactant, which is characterized in that the surfactant and the biological solution are brought into contact immediately before or during the purification of the macromolecules on the chromatographic material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.