Methods of determining the presence of double stranded nucleic acids in a sample
US6428959B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 6, 2000 |
| Grant date | Aug 6, 2002 |
| Priority date | — |
| Expiry date | Sep 6, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for determining the presence of double stranded nucleic acids in a sample are provided. In the subject methods, nucleic acids present in a fluid sample are translocated through a nanopore, e.g. by application of an electric field to the fluid sample. The current amplitude through the nanopore is monitored during the translocation process and changes in the amplitude are related to the passage of single- or double-stranded molecules through the nanopore. The subject methods find use in a variety of applications in which the detection of the presence of double-stranded nucleic acids in a sample is desired, e.g. in hybridization assays, such as Northern blot assays, Southern blot assays, array based hybridization assays, etc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.