Selection method for producing recombinant baculovirus
US6428960B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 30, 2000 |
| Grant date | Aug 6, 2002 |
| Priority date | — |
| Expiry date | Oct 30, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/60
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a novel method for producing recombinant baculoviruses expressing genes of interest by inserting a selectable marker gene, specifically the puromycin resistance (pac) gene, adjacent to the gene of interest into the baculovirus genome, and selecting for viruses that express the selectable marker. Selection of the recombinant virus in the presence of a selection condition, such as puromycin-containing cell culture media, simplifies virus production by eliminating the need for cloning, and testing the virus for gene expression. Such expression systems are readily adapted to an automated method for expression foreign genetic material in a high throughput manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.