Patent · US Expired

Nucleic acid molecules encoding histidine tags in three reading frames

US6428961B1 · kind B1 · utility

0Cited by
1References
24Claims
0Family size

Inventors

Key dates

Filing dateDec 8, 2000
Grant dateAug 6, 2002
Priority date
Expiry dateDec 8, 2020

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K2319/21
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes histidine tags in each of the three possible reading frames and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences 5′ or 3′ to the 3-frame His-tag DNA sequence.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.