LDL receptor signaling pathways

US6428967B1 · kind B1 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Joachim Herz · Austin, US
• Michael Gotthardt · Berlin, DE

Key dates

• Filing date: May 1, 2000
• Grant date: Aug 6, 2002
• Expiry date: May 1, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/92
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides methods and compositions for inducing and detecting signal transduction through LDL receptors, including specifically detecting a stress that alters a functional interaction of a low density lipoprotein (LDL) receptor binding polypeptide with an LDL receptor interaction domain, by (a) introducing a predetermined stress into a system which provides a physical interaction of an LDL receptor binding polypeptide with an LDL receptor intracellular binding polypeptide interaction domain, whereby the system provides a stress-biased interaction of the binding polypeptide and the interaction domain, wherein the absence of the stress, the system provides a unbiased interaction of the binding polypeptide and the interaction domain; and (b) detecting the stress-biased interaction of the binding polypeptide and the interaction domain, wherein the binding polypeptide is selected from SEMCAP-1, JIP-1, PSD-95, JIP-2, Talin, OMP25, CAPON, PIP4,5 kinase, Na channel brain 3, Mint1, ICAP-1 and APC subunit10.