Method for producing taxane-type diterpenes
US6428989B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 24, 1998 |
| Grant date | Aug 6, 2002 |
| Priority date | — |
| Expiry date | Aug 19, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a method for producing a taxane-type diterpene(s) by culturing cells of a taxane-type diterpene-producing plant, comprising one or both of the the following steps (a) and (b) to increase the ratio of those cell clusters suitable in size for diterpene production:(a) at least one operation for removing large cell clusters is conducted with a sieve and/or a filter during preculture of the cells to be supplied to main culture for the production of taxane-type diterpenes or at the time of cell transfer from one preculture to the subsequent preculture;(b) the cells are cultured under strong agitation conditions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.