cDNA, genomic, and predicted protein sequences of learning-induced kinases
US6428994B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 1, 1999 |
| Grant date | Aug 6, 2002 |
| Priority date | — |
| Expiry date | Oct 1, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/11022
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
To elucidate molecular mechanisms in learning and memory, the expression of mRNAs in brains of rabbitsundergoing eyeblink conditioning was analyzed. Infusion of the transcription inhibitor actinomycin D (ActD) into the cerebellar nuclei reversibly blocked learning but not performance of the CR. Differential display PCR (DD-PCR) analysis of cerebellar RNAs from trained and pseudo-trained rabbits identified a 207-bp band that was induced with learning. The fragment was used to isolate a cDNA from a &lgr;gt11 rabbit brain library containing a 1698-bp open-reading frame. The genomic sequence also has been obtained and is reported. The deduced amino acid sequence contains the KKIAMRE motif, which is conserved among cdc2-related kinases. These results suggest that there is a new category of cdc2-related kinases in the brain whose function may be important in learning and memory.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.