Replica amplification of nucleic acid arrays
US6432360B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 28, 1998 |
| Grant date | Aug 13, 2002 |
| Priority date | — |
| Expiry date | Aug 28, 2018 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00641
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules from which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.