Diagnostic applications of perlecan domain I splice variants
US6432636B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 26, 1997 |
| Grant date | Aug 13, 2002 |
| Priority date | — |
| Expiry date | Aug 26, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/2821
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An isolated polynucleotide that is a splice variant of perlecan formed by a partial or complete deletion of a number of exons within domain I of perlecan, the number of exons deleted from domain I numbering less than the number of exons in domain I. An isolated polynucleotide that is a splice variant of perlecan formed by an insertion of nucleotides within domain I of perlecan. A method of detection and/or quantitation of a splice variant of perlecan domain I gene in a sample, the method comprising making complimentary DNA (cDNA) from messenger RNA (mRNA) in the sample, amplifying portions of the cDNA corresponding to the perlecan gene or parts thereof and detecting and quantifying the amplified cDNA in order to detect or quantify the splice variant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.