Amplification of chromosomal DNA in situ
US6432650B1 · kind B1 · utility
4Cited by
5References
69Claims
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Key dates
| Filing date | Nov 2, 2000 |
| Grant date | Aug 13, 2002 |
| Priority date | — |
| Expiry date | Nov 2, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.
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