Peptide nucleic acid precursors and methods of preparing same
US6433134B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 5, 2000 |
| Grant date | Aug 13, 2002 |
| Priority date | — |
| Expiry date | Jan 5, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/047
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel and efficient syntheses create novel piperazinone intermediates which facilitate the production and use of PNAs. Such syntheses and the products enhance the feasibility of a system which permits the rapid identification of PNA oligomers useful as therapeutics, diagnostics and/or gene characterization tools. A first component of the system is a universal PNA library that most preferably incorporates one or more universal nucleotide bases into carefully selected positions within each oligomer species thereby providing the library with the screening ability of a much larger library. The second component of the system is a high throughput screening system that includes a number of assays designed to provide information on the binding activities of the different PNAs to a target nucleotide sequence (generally, a DNA or RNA sequence). The third component is a software system especially designed to provide rapid analysis of the data collected from the high throughput screening system and to determine therefrom the sequence base identities and sequence lengths of PNA oligomers most likely to bind to and appropriately affect the target molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.