Debranching enzymes and DNA sequences coding them, suitable for changing the degree of branching of amylopectin starch in plants

US6433253B1 · kind B1 · utility

Assignee

• Hoechst Schering Agrevo GmbH · DE

Inventors

• Jens Kossmann · Golm, DE
• Michael Emmermann · Michendorf, DE
• Ivar Virgin · Stockholm, SE

Key dates

• Filing date: Aug 6, 1999
• Grant date: Aug 13, 2002
• Expiry date: Aug 6, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/2451
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to DNA sequences which, on the codogenic strand, code plant debranching enzymes whose transcripts formed in transgenic plants code new proteins with the enzymatic activity of debranching enzymes which in transgenic plants reduce the degree of branching of amylopectin starch. The invention also relates to DNA sequences which on the codogenic strand code plant debranching enzymes whose transcripts formed in transgenic plants prevent the synthesis of proteins with the enzymatic activity of debranching enzymes, which in transgenic plants increases the degree of branching of amylopectin starch, and also to recombinant plasmids on which these DNA sequences are localized and which can be introduced into plant cells and plants.