Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6436675B1 · kind B1 · utility
213Cited by
30References
39Claims
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Key dates
| Filing date | Sep 28, 1999 |
| Grant date | Aug 20, 2002 |
| Priority date | — |
| Expiry date | Sep 28, 2019 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of providing shuffling libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.