Methods for defining cell types
US6441269B1 · kind B1 · utility
5Cited by
2References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 9, 2000 |
| Grant date | Aug 27, 2002 |
| Priority date | — |
| Expiry date | May 9, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.