Production and use of normalized DNA libraries
US6444426B1 · kind B1 · utility
27Cited by
1References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 9, 1999 |
| Grant date | Sep 3, 2002 |
| Priority date | — |
| Expiry date | Nov 9, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.