Detection and treatment of duplex polynucleotide damage

US6444661B1 · kind B1 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Jacqueline K. Barton · New York, US
• Brian Jackson · Pasadena, US
• Brian P. Hudson · Pasadena, US

Key dates

• Filing date: Nov 23, 1999
• Grant date: Sep 3, 2002
• Expiry date: Nov 23, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07F15/008
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Duplex polynucleotides containing damage or errors are detected with hindered intercalating compounds which are capable of intercalating only in the presence of such damage or error. Conditions characterized by the presence of polynucleotide errors or damage are treated with such compounds that are capable of catalyzing polynucleotide cleavage with light. Suitable compound include compounds of the formula:Rh(R1)(R2)(R3)3+where R1 and R2 are each independently aryl, heteroaryl, substituted aryl or substituted heteroaryl of 1 to 5 rings, and R3 is a group of the formula where x and z are each independently an integer from 1 to 4 and y is an integer from 1 to 2, and R4, R5, and R6 are each independently H—, halo, HO—, H2N—, CN—, O2N—, HS—, O3S—, O3SO—, —COOH, —CONH2, R, RO—, RNH—, RaRbN—, RO3S—, RO3SO—, —COOR, —CONHR, or —CONRaRb, where R, Ra and Rb are each independently lower alkyl, cycloalkyl, lower alkenyl, lower alkynyl, or phenyl, or two R4, R5, or R6 together form a fused aryl ring.