In vitro assay method for the study of brain aging
US6447988B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Jan 22, 1997 |
| Grant date | Sep 10, 2002 |
| Priority date | — |
| Expiry date | Jan 22, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5082
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Cultured brain slices are treated with a free radical generator, in the presence of a lysosomal enzyme inhibitor (specifically an inhibitor of two cathepsins). The treated brain slices rapidly develop autofluorescent lipofuscin granules—a universal feature of brain aging. Other correlates of the aged brain are also induced by this treatment, thereby providing an in vitro model for (1) the study of brain aging; (2) assessment of anti-brain aging drugs; and (3) therapeutics directed at the clinical condition referred to as neuronal ceroidlipofuscinosis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.