Pta LDHA double mutant Escherichia coli SS373 and the method of producing succinic acid therefrom
US6448061B1 · kind B1 · utility
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4Claims
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Key dates
| Filing date | Jan 27, 2000 |
| Grant date | Sep 10, 2002 |
| Priority date | — |
| Expiry date | Jan 27, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/19
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a mutant Escherichia coli SS373 and the production of succinic acid by using the above strain. In detail, a novel E. coli SS373 (W3110 pta::Tn10 ldhA::Km) with the deficiency in the acetate and lactate forming pathways was constructed by genetic engineering technique. An aerobically grown SS373 was then cultured by means of the anaerobic condition shift during the succinate producing stage, which resulted in the efficient production of succinic acid with a higher yield.
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