Method for chemically acellularizing a biological tissue sample
US6448076B2 · kind B2 · utility
85Cited by
22References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 29, 2001 |
| Grant date | Sep 10, 2002 |
| Priority date | — |
| Expiry date | Jun 29, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/92
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for chemically acellularizing a biological tissue sample, such as a peripheral nerve, is provided. The method includes disrupting the cell membranes of the biological tissue sample, and then denaturing intracellular proteins within the cells of the tissue sample and removing the denatured proteins from the cells while preserving the extracellular matrix to produce an acellularized tissue construct.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.