Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
US6448407B1 · kind B1 · utility
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Key dates
| Filing date | Nov 1, 2000 |
| Grant date | Sep 10, 2002 |
| Priority date | — |
| Expiry date | Nov 1, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/533
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Substantially pure atropisomers of xanthene compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.