3′-exonuclease, production and use thereof

US6451307B1 · kind B1 · utility

Assignee

• Aventis Research & Technologies GmbH & Co. · DE

Inventors

• Thomas Neumann · Frankfurt am Main, DE
• Christoph Huls · Wackernheim, DE
• Karl-Christian Gallert · Karben, DE
• Anders Virtanen · Uppsala, SE
• Jonas Aström · Uppsala, SE
• Javier Martinez · Uppsala, SE
• Ann-Charlotte Thuresson · Uppsala, SE
• Yanguo Ren · Uppsala, SE

Key dates

• Filing date: Dec 15, 2000
• Grant date: Sep 17, 2002
• Expiry date: Dec 15, 2020

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to a poly(A)-specific 3′-exonuclease activity which can be obtained by chromatographically purifying a crude extract of animal or human cells and to its use for deadenylating 3′-poly(A) tails of nucleic acids and as a pharmaceutical or diagnostic agent, or for identifying functional interactors.