Sesquiterpene synthases from grand fir (Abies grandis), and methods of use

US6451576B1 · kind B1 · utility

Assignee

• Washington State University Research Foundation · US

Inventors

• Rodney B. Croteau · Pullman, US
• Jorg Bohlmann · Jena, DE
• John E. Crock · Troy, US
• Christopher L. Steele · Ardmore, US

Key dates

• Filing date: May 24, 2001
• Grant date: Sep 17, 2002
• Expiry date: May 24, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8279
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

cDNAs encoding, E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No:24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or ,&ggr;-humulene synthase. Thus, systems and methods are provid…