Cell fusion assays for the identification of antiviral compounds, and systems and kits for practicing the same
US6451598B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 7, 2001 |
| Grant date | Sep 17, 2002 |
| Priority date | — |
| Expiry date | Feb 7, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56983
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Cell fusion assays for identifying antiviral compounds are provided. In the cell fusion assays of the subject invention, a first cell that stably expresses on its surface an envelope protein of an enveloped virion and a second cell that stably expresses a receptor for the envelope protein on its surface are employed. The first and second cells each contain one component of a two component Tat reporter system that produces a detectable product in the presence of cell fusion. In practicing the subject screening methods, the first and second cells are first contacted with each other under cell fusion conditions in the presence of a candidate inhibitory agent. Next, the presence or absence of the detectable product is detected. Finally, the inhibitory activity of the candidate agent is derived from the presence or absence of the detectable product. Also provided are high throughput embodiments of the subject methods. In addition to the subject methods, systems and kits for performing the subject methods are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.