Capping and de-capping during oligonucleotide synthesis
US6451998B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 18, 1999 |
| Grant date | Sep 17, 2002 |
| Priority date | — |
| Expiry date | Oct 18, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method of capping a hydroxy group of a moiety, comprising coupling the moiety to a phosphor or phosphite derivative of a protected alcohol, so as to form the corresponding phosphate or phosphite between the hydroxy and phosphor or phosphite groups. The hydroxy group may be later de-capped by hydrolyzing the resulting compound to deprotect the protected alcohol and cleave the phosphate from the moiety so as to regenerate the hydroxy group of the moiety. The method has particular application to fabrication of addressable polynucleotide arrays and allows failed sequences, as well as inter-feature regions, to be left with a free hydroxy group at the ends of the molecules (failed sequences or linkers) at such locations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.