Method and apparatus for deriving separate images from multiple chromogens in a branched image analysis system
US6453060B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 29, 1999 |
| Grant date | Sep 17, 2002 |
| Priority date | — |
| Expiry date | Jun 29, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1472
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system for collecting images at multiple wavelengths for image analysis of biological specimens. The process separates contrasts for images of a biological specimen prepared with a chromogen. Light wavelengths matched with each chromogen optimize separation for each image. Computing attenuation coefficients and extinction coefficients for each chromogen provides for determination of the concentration of each chromogen. A chromogen separator allows for subtracting predetermined chromogens to generate new images and aid in identification of cell morphology. The system can perform the image analysis of the new images using image processing techniques such as segmentation, feature calculation and object classification. At each stage of image processing, data from each of the new images may influence the processing of the other images.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.