Detection of chromosome copy number changes to distinguish melanocytic nevi from malignant melanoma

US6455258B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Boris Bastian · San Francisco, US
• Daniel Pinkel · Lafayette, US

Key dates

• Filing date: Apr 13, 2001
• Grant date: Sep 24, 2002
• Expiry date: Apr 13, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides for methods of distinguishing melanocytic nevi, such as Spitz nevi, from malignant melanoma. The methods comprise contacting a nucleic acid sample from a patient with a probe which binds selectively to a target polynucleotide sequence on a chromosomal region such as 11p, which is usually amplified in Spitz nevi. The nucleic acid sample is typically from skin tumor cells located within a tumor lesion on the skin of the patient. Using another probe which binds selectively to a chromosomal region such as 1q, 6p, 7p, 9p, or 10q, which usually show altered copy number in melanoma, the method can determine that those tumor cells with no changes in copy number of 1q, 6p, 7p, 9p, or 10q, are not melanoma cells but rather Spitz nevus cells. The finding of amplifications of chromosome 11p would be an additional indication of Spitz nevus.