Hydrolytic enzyme substrates and assay method
US6455268B1 · kind B1 · utility
5Cited by
1References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 3, 2000 |
| Grant date | Sep 24, 2002 |
| Priority date | — |
| Expiry date | Nov 5, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Compounds of the invention have the structure D—L—A*, where L is a linking moiety that maintains D and A in a configuration compatible with FRET, D is a fluorescent donor moiety capable of causing an acceptor moiety to fluoresce by FRET, and A* is a fluorescent acceptor moiety capable of accepting energy from the donor moiety by FRET and fluorescing only after cleavage of one or more labile groups to provide acceptor A. These compounds are useful as fluorescent substrates for detection of enzymes in vivo.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.