Detection of biologically active molecules by use of pre-activated cell-based biosensors in liquid-based separation systems
US6455303B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 2, 1999 |
| Grant date | Sep 24, 2002 |
| Priority date | — |
| Expiry date | Dec 2, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/802
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method for detection of receptor- or ion channel-modulators (e.g., antagonists, blockers, etc.). The method comprises fractionating a sample by using a liquid-based separation means (e.g., such as a capillary electrophoresis device), and feeding the fractions containing the modulators directly to a biosensor. The biosensor is activatable by a receptor agonist and produces a measurable response as a result of this activation. Preferably, the agonist is fed to the biosensor through the liquid-based separation means, together with fractions containing the modulators (e.g., antagonists or blockers). The presence of a modulator is detected by monitoring a change in the response of the biosensor, e.g., by measuring changes in electrical properties of the biosensor (such as through patch clamp analysis). In one aspect, the biosensor is periodically resensitized by delivering pulses of receptor agonist to the biosensor for short period of time which are separated by other periods when no agonist is delivered to the biosensor. The invention further provides a system for performing the method. The system facilitates sequential patch clamp recordings of cell-based bio…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.