Method for producing a heterologous secreted protein from chinese hamster ovary cells grown on microcarriers
US6458565B1 · kind B1 · utility
9Cited by
1References
13Claims
0Family size
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Key dates
| Filing date | Sep 1, 2000 |
| Grant date | Oct 1, 2002 |
| Priority date | — |
| Expiry date | Sep 1, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2531/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for producing a heterologous secreted protein from transformed CHO cells grown on microcarriers in a serum-free cell culture medium. This method avoids a production lag phase when switching from a serum-containing to a serum-free environment, a problem observed in the mammalian adherent cell culture production of secreted proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.