Method for the production of baccatin-III by enzymatic synthesis

US6458568B1 · kind B1 · utility

Assignee

• Institut fuer Bioanalytik, Umwelt-Toxikologie und Biotechnologie Halle GmbH · DE

Inventors

• Konrad Glund · Halle (Saale), DE
• Matthias Hoffmann · Hendersonville, US
• Wolfram Weckwerth · Berlin, DE
• Rainer Zocher

Key dates

• Filing date: May 13, 1999
• Grant date: Oct 1, 2002
• Expiry date: May 13, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P17/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for the continuous synthesis of baccatin-III is performed by means of the enzymatic acetylations of 10-deacetyl baccatin-III (10-DAB) in an enzyme reactor. This objective is accomplished by a method, for which an aqueous reaction batch is used, which contains a partially purified or a very pure acetyl transferase from a Taxus species and synthesis components, especially 10-DAB, acetyl compounds or optionally an acetyl coenzyme A, which can be regenerated, a protective colloid and optionally a buffer substance in solution. The solution is separated by a semipermeable membrane from an organic solvent, which serves as extraction agent for baccatin-III, the reaction batch being regenerated at regular intervals in an aqueous buffer system and the solvent continuously being exchanged. By conducting the reaction, the product can be removed continuously from the reaction batch for the partial synthesis of taxol and taxoter. By the above method, achievable yields are increased significantly.