Compositions and methods for the targeted removal of a nucleotide sequence from the genome of a plant
US6458594B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 12, 1999 |
| Grant date | Oct 1, 2002 |
| Priority date | — |
| Expiry date | Nov 12, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/53
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions to remove a nucleotide sequence of interest in a plant and plant cell are provided. In particular the methods of the invention comprise providing a plant cell having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by non-identical recombination sites and introducing into the plant cell a chimeric RNA-DNA oligonucleotide molecule. The chimeric RNA-DNA oligonucleotide is capable of recognizing and implementing a nucleotide conversion in one of the non-identical recombination sites so as to create two identical recombination sites. An appropriate recombinase is provided which excises the sequences between the two identical recombination sites.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.