Methods for measuring cellular proliferation and destruction rates in vitro and in vivo
US6461806B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 15, 1999 |
| Grant date | Oct 8, 2002 |
| Priority date | — |
| Expiry date | Nov 15, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/60
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to methods for measuring the proliferation and destruction rates of cells by measuring deoxyribonucleic acid (DNA) synthesis and/or destruction. In particular, the methods utilize non-radioactive stable isotope labels to endogenously label DNA synthesized through the de novo nucleotide synthesis pathway in a cell. The amount of label incorporated in the DNA is measured as an indication of cellular proliferation. The decay of labeled DNA over time is measured as an indication of cellular destruction. Such methods do not involve radioactivity or potentially toxic metabolites, and are suitable for use both in vitro and in vivo. Therefore, the invention is useful for measuring cellular proliferation or cellular destruction rates in humans for the diagnosis, prevention, or management of a variety of disease conditions in which cellular proliferation or cellular destruction is involved. The invention also provides methods for measuring proliferation or destruction of T cells in a subject infected with human immunodeficiency virus (HIV) and methods of screening an agent for a capacity to induce or inhibit cellular proliferation or destruction. In addition, th…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.