Method for chromosomal rearrangement by consecutive gene targeting of two recombination substrates to the deletion endpoints
US6461818B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 19, 2000 |
| Grant date | Oct 8, 2002 |
| Priority date | — |
| Expiry date | Apr 19, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention involves the creation of defined chromosomal deficiencies, inversions and duplications using Cre recombinase in ES cells transmitted into the mouse germ line. These chromosomal reconstructions can extend up to 3-4 cM. Chromosomal rearrangements are the major cause of inherited human disease and fetal loss. Additionally, translocations and deletions are recognized as major genetic changes that are causally involved in neoplasia. Chromosomal variants such as deletions and inversions are exploited commonly as genetic tools in organisms such as Drosophila. Mice with defined regions of segmental haploidy are useful for genetic screening and allow accurate models of human chromosomal diseases to be generated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.