Clostripain catalyzed amidation of peptides

US6461834B1 · kind B1 · utility

Assignee

• Bionebraska, Inc. · US

Inventors

• Dan Dormady · Omaha, US
• Jay Stout · Lincoln, US
• Daniel Strydom · Eagle, US
• Barton Holmquist · Lincoln, US
• Fred W. Wagner · Walton, US

Key dates

• Filing date: Dec 16, 1998
• Grant date: Oct 8, 2002
• Expiry date: Dec 16, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/06
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a method of producing a polypeptide having a C-terminal &agr;-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an &agr;-carboxamide group (C-terminal “Arg-NH2”). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.